This depends on your specific hotel. If your are staying in downtown San José or in the center of Puerto Viejo then around 6:30 am. If you an outside of downtown San José or in the Southern Caribbean beaches ( like Cocles ) expect to be picked up at around 6:20 am.
In the Arenal Volcano we have a single pick up time: 5:30 am.
This pick up times apply both for our river trips and Tortuguero.